Rapamune®
Sirolimus Monitoring by LC – MS/MSTMS

BioScience Laboratory offers a reference lab service for the therapeutic drug monitoring of Rapamycin (Sirolimus, Rapamune® - Wyeth) for transplant programs. We utilize high performance liquid chromatography coupled to electro-spray ionization tandem mass spectrometry (HPLC-ESI-MS/MS or LC-MS/MS) as the definitive quantitative method to accurately determine blood levels of rapamycin. Tandem mass spectrometry has been demonstrated in numerous studies to be the method of choice for therapeutic drug monitoring of rapamycin. In addition, the multiplex capability of LC-MS/MS allows for simultaneous measurement of other prescribed immunosuppressants, Tacrolimus (Prograff®), Cyclosporin A (Sandimmune® & Neoral®, and Everolimus (Certican®), if desired. Unfortunately, high equipment costs and the requirement for highly skilled personnel have prevented implementation of this superior technology in many centers. TMS BioScience has state-of-the-art instrumentation systems and a well trained and experienced staff to offer this service to transplant programs. By use of overnight courier services and dedicated assay procedures, our turn-around time is suitable for acute therapeutic management as well as routine monitoring of transplant patients. Result reports are available within 24 hours of receipt of blood samples. Our staff scientists have years of experience in clinical applications of tandem mass spectrometry and our senior scientists are recognized leaders in clinical mass spectrometry.

Prograff ®
Tacrolimus Monitoring by LC – MS/MS

TMS BioScience Laboratory offers a reference lab service for the therapeutic drug monitoring of Tacrolimus (Prograf® - Astellas) for transplant programs. We utilize high performance liquid chromatography coupled to electro-spray ionization tandem mass spectrometry (HPLC-ESI-MS/MS or LC-MS/MS) as the definitive quantitative method to accurately determine blood levels of Tacrolimus. Tandem mass spectrometry has been demonstrated in numerous studies to be the method of choice for therapeutic drug monitoring of Tacrolimus; tandem mass spectrometry does not react with drug metabolites and is not affected by liver function or sample preparation as is the standard immunoassay test used. The immunoassay is nonspecific and has been shown to give erroneously high drug levels in a variety of situations which may lead to under dosing and risk of organ rejection. In addition, the multiplex capability of LC-MS/MS allows for simultaneous measurement of other prescribed immunosuppressants, Sirolimus (Rapamune®), Cyclosporin A (Sandimmune® & Neoral®, and Everolimus (Certican®), if desired. Unfortunately, high equipment costs and the requirement for highly skilled personnel have prevented implementation of this superior technology in many centers. TMS BioScience has state-of-the-art instrumentation systems and a well trained and experienced staff to offer this service to transplant programs. By use of overnight courier services and dedicated assay procedures, our turn-around time is suitable for acute therapeutic management as well as routine monitoring of transplant patients. Result reports are available within 24 hours of receipt of blood samples. Our staff scientists have years of experience in clinical applications of tandem mass spectrometry and our senior scientists are recognized leaders in clinical mass spectrometry.

Cyclosporin A
Cyclosporin A Monitoring by LC – MS/MS

TMS BioScience Laboratory offers a reference lab service for the therapeutic drug monitoring of Cyclosporin A (Sandimmune®, Neoral® - Novartis) for transplant programs. We utilize high performance liquid chromatography coupled to electro-spray ionization tandem mass spectrometry (HPLC-ESI-MS/MS or LC-MS/MS) as the definitive quantitative method to accurately determine blood levels of Cyclosporine A. Tandem mass spectrometry has been demonstrated in numerous studies to be the method of choice for therapeutic drug monitoring of Cyclosporine A; tandem mass spectrometry does not react with drug metabolites and is not affected by liver function or sample preparation as is the standard immunoassay test used. The immunoassay test is nonspecific and has been shown to give erroneously high drug levels in a variety of situations which may lead to under dosing and risk of organ rejection. In addition, the multiplex capability of LC-MS/MS allows for simultaneous measurement of other prescribed immunosuppressants, Sirolimus (Rapamune®), Cyclosporin A (Sandimmune® & Neoral®, and Everolimus (Certican®), if desired. Unfortunately, high equipment costs and the requirement for highly skilled personnel have prevented implementation of this superior technology in many centers. TMS BioScience has state-of-the-art instrumentation systems and a well trained and experienced staff to offer this service to transplant programs. By use of overnight courier services and dedicated assay procedures, our turn-around time is suitable for acute therapeutic management as well as routine monitoring of transplant patients. Result reports are available within 24 hours of receipt of blood samples. Our staff scientists have years of experience in clinical applications of tandem mass spectrometry and our senior scientists are recognized leaders in clinical mass spectrometry.